Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILKIEDLVMSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKVSLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLT-----SGDLVL-EIIGSVRSLLTCERVALNFLQHLSGIASMTAAYVEALGDDCIK--VFDTRKTTPNLRLFEKYAVRVGGGYNHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGADIIMLDNMSLEQIEQAITLIAGRSR---IECSGNIDMTTISRFRGLAIDYVSSGSLTHSAKSLDFSMKGLTYLDV 3C2F Chain:A ((21-291)) -----------------------------LSEDVPSFDFGGYVVGSDLKEA--NLYCKQDGMLCGVPFAQEVFNQCELQVEWL----FKEGSFLEPSKNDSGKIVVAKITGPAKNILLAERTALNILSRSSGIATASHKIISLARSTGYKGTIAGTRKTTPGLRRLEKYSMLVGGCDTHRYDLSSMVMLKDNHIWATGSITNAVKNARAVCGFAVKIEVECLSEDEATEAIEAGADVIMLDN------------------HFLLECSGGLNL------LCDDIDIYSTSSIHQGTPVIDFSLK-------

General information: TITO was launched using: RESULT: Template: 3C2F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1077 -18293 -16.98 -78.51 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -16.98 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.460

(partial model without unconserved sides chains): PDB file : Tito_3C2F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3C2F-query.scw PDB file : Tito_Scwrl_3C2F.pdb: