Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQNLPIIAVLYMILSMVSYQISASFAKQLIAALDPLTVTILRLC---FAAVIVAVMFRSWKIWSRLPYLKWRD----LLMYCASLGLMNILFYTSLGKLPQGIAVGLEFIGPL--GLALVSIKQKSEYIWVAFAMLG-----------IALMVPWQDPTQHHFSYVGA---ACALGAGLC------WAFYIYFGQKVVTQNIGMHALTIGIT--VSALALLPIGIWYN------GPALLD---------------TQYWGKALLIAVLATAIPYA--------------------LDLMALKQLNKLTYGTLTSLAPALAALTGFLLLHEEISMLQW---VALACV-------MLASIGVTLRSKTAS--------- 5JX2 Chain:A ((20-387)) -RDKPLVFF---------NRQPSDPGKVDMAAMNWNDKTYYVGFDAKFGGSIQGKMILDFLASSESSVDRNGDGIIGYVLCIGDVGHNDSKVRTEGIRRALGTWTGSSDPGQAKEGQAVVGGKSYKVVELEGKAMTGTDGSTWNTNSATESMGSWVAKFADKIDLVISNNDGMAMGCLQASNYPRGLPIFGYDANADAVESVGKGELTGTVSQNVDAQAVAVLQIIRNLLDGSSGEDVVANGISRPDAHGNISAPVQYWEDVKAIMADNSEVTSANWKEYTRGARDAGVRQVSAPTKKVLLTVHNASNDFLASAYLPALKHYA--PLLNVDLTVVQGDGQNELSCLDKFTNLDMFDAFAVNMVKTNSGADYTDKLKY

General information: TITO was launched using: RESULT: Template: 5JX2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1495 -132172 -88.41 -482.38 target 2D structure prediction score : 0.28 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -88.41 2D Compatibility (Sec. Struct. Predict.) : 0.28 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.109

(partial model without unconserved sides chains): PDB file : Tito_5JX2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JX2-query.scw PDB file : Tito_Scwrl_5JX2.pdb: