Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDITLAPVQPDQPTHLMATYGRQAISFVRGRGAYLYTQDGTEYLDALTGIAVCGLGHTHPTIAEAIAEQAATLVHTSN--LFEIPWQTAAAQKLAEVSG--MEEIFFSNSGAESNEGAIKIARKFGTQQGISLPKIIVAEQSFHGRTLATLSATGNKKVQDGFAPLVEGFIRVPFGDIEAIQEAALQHPDIVAILIEPIQGEGGINTAPQGFSYLEEVRQICNQHNWLMMLDEIQTGNGRTGKYFAYQHTNIIPDVLTTAKGLGNG-FPVGAVMTQGKAVGLLGP-----GSHGSTYGGTVLGSRVVYTVIDTIQKENAVENAAVVGNYIVDQLRAQLSD---KNVQVRGFGMMIGIQLPKDC---------------------------------AELVAIARDEYKLIINVTA--GSVVRLLPPINMTQAQADILLECLVALITNYL 4PPM Chain:B ((414-848)) ----------------------RCDNVFRSAAGTQLYDDAGEAFLDMVAGYGCLNLGHNPQPVVNALKNYLDAQGPNFIQYISIPEQTAKLAEVLCRLAPGNMGRVFFSNSGTEAVEAAMKIAKAST-----GKPGIAYLRNSYHGKTLGALSITGRDKHRRYFTPLLDAMVEVPFGDLAALREALN-REDVGALMIEPIQGEGGVH--IPPAGYLQAVQQLCRETGVLLMVDEVQTGLGRTGKLFACEWDGIEPDVLMLSKSLSGGLIPIGATLCRADLWQKAYGTADRFLVHSSTYGGGNLASVVALSALREILAQDLVGHAERMGAYFKQALSE-IAARYPFVSEVRGRGLMLGIQFD---WHTTWKFLPDPVQAHLRAAMDRMEQALGEMFCMKFVTKLCQD-HKILTFITANSSTVIRIQPPLIISKAEIDRFVGAFATVCEEL-

General information: TITO was launched using: RESULT: Template: 4PPM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1863 -6908 -3.71 -18.87 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -3.71 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.439

(partial model without unconserved sides chains): PDB file : Tito_4PPM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PPM-query.scw PDB file : Tito_Scwrl_4PPM.pdb: