Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDKNIGGMKMIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGTTGESSTLTQSEEEQILQLTVKKVAG-RVPVIAGAGTNNTKETIEKAKHFASLGADALLVITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNL-AEHPNIIGLKEASGDMAYVMDAARLIGEE--FFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELVKDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDL-PSGPLRLPLVSLSKEKKKQLAQLLLKERTVEK 5F1V Chain:D ((3-293)) ---NI-----IIGAMTALITPF-KNGKVDEQSYARLIKRQIENGIDAVVPVGTTGESATLTHEEHRTCIEIAVETCKGTKVKVLAGAGSNATHEAVGLAKFAKEHGADGILSVAPYYNKPTQQGLYEHYKAIAQSVDIPVLLYNVPGRTGCEISTDTIIKLFRDCENIYGV-EASGNIDKCVD---LLAHEPRMMLISGEDAINYPILSNGGKGVISVTSNLLPDMISALTHFALDENYKEAKKINDELYNINKILFCESNPIPIKTAMYLAGLIESLEFRLPLCSPSKENFAKIEEVMKKYK----

General information: TITO was launched using: RESULT: Template: 5F1V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1671 -42128 -25.21 -147.82 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain D : 0.82 3D Compatibility (PKB) : -25.21 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.606

(partial model without unconserved sides chains): PDB file : Tito_5F1V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5F1V-query.scw PDB file : Tito_Scwrl_5F1V.pdb: