Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMSLHRYKGKYMSNKRIAIIGAGPAGLMAAEVLSQYAYEIDVFEQKPSAARKFLM-AGKTGLNISHAEPLPQFIER--YDHTEWLKPWVEKWDAVWIQKWMKSLGIESYVGTSGRIFPVEMKAAPLLRAWLKRLAEQQVKFFYRHRCINL--QGTTVTLENQNNQSTETFTQQYDAVILACGAVSWSQLGSDGAWQQWMDQSSI---EPFQASNAGVLHTWSAFMESCFGEPLKRINAWVEPSQVTHGDIVITHYGLESGVIYKLGRALREQQKQGKSLNLFLDLLPD----VELTQLVKKLQASKKQSLTNIWRKA-GLDTAKINLIREVVDKALWSQPETMAQKMKALQIKLDGFRPIEEAISCAGGVKQDALTESLELKQTSGVFCCGEMLDWDAPTGGYLLTACFATGRAAGEGVHSFLEK 4J33 Chain:A ((22-411)) ------------MSESVAIIGAGLVGCLAALAFSKEGYNVTLYDFRQDPRLDTTKNKNLKSINLAISARGIDALKSIDPDACEHILQDMIPMKGRMIHDLKGRQESQLY---GEAINSIN--RSVLNNSLLDELEKSTTELKFGHKLVKIEWTDDKQICHFAIG---TPHTEKYDFVIGCDGA--YSATRSQMQRKVEMDFSQEYMNLRYIELYIPPTEEFKPNYGGNFAIAPDHLHIWPRHKFMLIALANSDG-SFTSTFFGSKDQISDLITSKSRVREFLIENFPDIINIMDLDDAVKRFITYPKESLVCVNCKPYDVPGGKAILLGDAAHAMVPFYGQ-------GMNCGFEDVRILMALLKKHSGDRSRAFTEYTQTRHKDLVSITELAKRNYKEMSHDVTSKRFLLRK------------

General information: TITO was launched using: RESULT: Template: 4J33.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2080 19767 9.50 53.42 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 9.50 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.219

(partial model without unconserved sides chains): PDB file : Tito_4J33.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4J33-query.scw PDB file : Tito_Scwrl_4J33.pdb: