Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTELQDIDFVELSDEEMHLYSRQILLDG-W---DIEAQEKLKLANVLIVGAGGIGCSSAELLARAGVGKITLIDADTIEISNLQRQIAFGHEDIGRYKAEVLAKRLQKINPYICVEYFNERLDEHNIDKLVEHQDVVLDGCDNF-TTRYLVNAACKKHQVALISASAIGFQAQMFMVE-G-DSACYECLFPKEQHA----N-------EGLRCAESGVLATTPVMIASLQAHHTLLYLGLNRTP-LKQKLLLWDGLNMTQRIVSFDKDINCPFCQAS 3H5A Chain:C ((84-349)) -----------NNSTENNRYSRNFLHYQSYGANPVLVQDKLKNAKVVILGCGGIGNHVSVILATSGIGEIILIDNDQIENTNLTRQVLFSEDDVGKNKTEVIKRELLKRNSEISVSEIALNINDYTDLHKVPEADIWVVSADHPFNLINWVNKYCVRANQPYINAGYVNDIAVFGPLYVPGKTGCYECQKVVADLYGSEKENIDHKIKLINSRFKPATFAPVNNVAAALCAADVIKFIGKYSEPLSLNKRIGIWSDEIKIHSQNMGRSPVCSVCGNR

General information: TITO was launched using: RESULT: Template: 3H5A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1212 8424 6.95 34.11 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain C : 0.68 3D Compatibility (PKB) : 6.95 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.426

(partial model without unconserved sides chains): PDB file : Tito_3H5A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3H5A-query.scw PDB file : Tito_Scwrl_3H5A.pdb: