Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGHSVALNFADGKTFFISVNKDELLLDAAVRQGINLPLDCREGVCGTCQGQCETGIYEQEYVDEDALSERDLAERKMLACQTRVKSDAAFYFDHDSAICNAGDTLKIETQVTAVELVSETTAILHLDASNHAEQLQFLPGQYARLQIPDT-------------------------------EDWRSYSFANRPNATNQLQFLIRL---------LPDGVMSNYLRDRCQVGQSLLIEAPLGSFYLREVERPLVFVAGGTGLSAFLGMLDNLVEQP-NSPTVQFYYGVNSETDLCEQQRLHAYAEQLPNFSYHPIVTKAT--ETWQGKAGYIHEHLNKDQ-L---AEQAFDMYLCGPPPMIEAVKNWLDEQALQNYRIYSEKFLQSNTSR 4U9U Chain:A ((3-281)) -------------------------------------------------------------------------------------------------------VKKWECTVISNDNKATFIKELKLAIPDG-ESVPFRAGGYIQIEAPAHHVKYADFDVPEKYRGDWDKFNLFRYESKVDEPIIRAYSMANYPEEFGIIMLNVRIATPPPNNPNVPPGQMSSYIWS-LKAGDKCTISGPFGEFFAKDTDAEMVFIGGGAGMAPMRSHIFDQLKRLKSKRKMSYWYGARSKREMFYVEDFDGLAAENDNFVWHCALSDPQPEDNWTGYTGFIHNVLYENYLKDHEAPEDCEYYMCGPPMMNAAVINMLKNLGVEEENILLDDFGG-----

General information: TITO was launched using: RESULT: Template: 4U9U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1185 -15694 -13.24 -67.64 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -13.24 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.510

(partial model without unconserved sides chains): PDB file : Tito_4U9U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4U9U-query.scw PDB file : Tito_Scwrl_4U9U.pdb: