Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIENDFS-DWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLESVTKG 2L4Q Chain:A ((26-113)) ----------------KPVLVDFWATWCGPCKMVAPVLEEIATERATDLTVAKLDVDTNPETARNFQVVSIPTLILFKDGQPVKRIVGAK--GKAALLRELSDVVP-

General information: TITO was launched using: RESULT: Template: 2L4Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 353 -18064 -51.17 -207.63 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -51.17 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.563

(partial model without unconserved sides chains): PDB file : Tito_2L4Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2L4Q-query.scw PDB file : Tito_Scwrl_2L4Q.pdb: