Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAG------KNP-ILVGILKGSIPFMAELVK-----HIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDIIDTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDYKENYRNLPYIGVLKEEVYSN 5JV5 Chain:A ((19-198)) --------VLFTEAELHTRMRGVAQRIADDYSNCNLKPLENPLVIVSVLKGSFVFTADMVRILGDFGVPTRVE--FLR--------------------LCDIRGKHVLVLEDILDTALTLREVVDSLKKSEPASIKTLVAIDKPGGRKIPFTAEYVVADVPNVFVVGYGLDYDQSYREVRDVVILKPSVY--

General information: TITO was launched using: RESULT: Template: 5JV5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 696 -21451 -30.82 -144.94 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -30.82 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.589

(partial model without unconserved sides chains): PDB file : Tito_5JV5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JV5-query.scw PDB file : Tito_Scwrl_5JV5.pdb: