Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDIEALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTALNRLNLKIKLYKEGKF 5CRL Chain:B ((8-119)) LTIGVFAKAAGVNVETIRFYQRKGLLL---------RRYGEADVTRVRFVKSAQRLGFSLDEIAELLRLEDG--THCEEASSLAEHKLKDVREKMADLARMEAVLSELVCACHA---

General information: TITO was launched using: RESULT: Template: 5CRL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 237 -41577 -175.43 -403.66 target 2D structure prediction score : 0.81 Monomeric hydrophicity matching model chain B : 0.73 3D Compatibility (PKB) : -175.43 2D Compatibility (Sec. Struct. Predict.) : 0.81 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.717

(partial model without unconserved sides chains): PDB file : Tito_5CRL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5CRL-query.scw PDB file : Tito_Scwrl_5CRL.pdb: