Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILKIEDLVMSIISTDLTPFQIDDTLKAALREDV-----HSEDYSTNAIFDHHGQAKVSLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLLTCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGYNHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGADIIMLDNMSLEQIEQAITLIAGRS---RIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAKSLDFSMKGLTYLDV 1QAP Chain:A ((8-296)) -------DDRRDALLERINLDIPAAVAQALREDLGGEVDAGNDITAQL-LPADTQAHATVITREDGVFCGKRWVEEVFIQLAG-DDVRLTWHVDDGDAIHANQTVFELQGPARVLLTGERTALNFVQTLSGVASEVRRYVGLLAGTQTQLLDTRKTLPGLRTALKYAVLCGGGANHRLGLTDAFLIKENHIIASGSVRQAVEKAFWLHPDVP-VEVEVENLDELDDALKAGADIIMLDNFNTDQMREAVKRVN---GQARLEVSGNVTAETLREFAETGVDFISVGALTKHVRALDLSMRFC-----

General information: TITO was launched using: RESULT: Template: 1QAP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1350 2028 1.50 7.22 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : 1.50 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.488

(partial model without unconserved sides chains): PDB file : Tito_1QAP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QAP-query.scw PDB file : Tito_Scwrl_1QAP.pdb: