Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQDTPLIIGSRSFQSRLLVGTGKYKDLNETDLAIQASGAEIVT----VAIRRVNIGQNPDQPNLLSVIPPEKYTILPNTAGCFD---ADSAVRTCMLARALLDGHNLVKLEVLGDEKTLYPNVTETLKAARTLIDDGFEIMVYTSDDPIIAQELESMGCVAI--MPLGSLIGSGLGIL--NPHTISIIKENA-----KVPVLVDAGVGTASDAAIAMELGCDGVLMNTAIAAAQNPILMASAMKKAVEAGREAFLAGRMPRKRMANASSPETGYFFK 1W0M Chain:H ((2-224)) -RLPILIINFKAYGE--AAGKRAVELAKAAERAARELGVNIVVAPNHLELGLVSQS--VDIPVYAQGADVEAG-------GAHTAHVSLENIKEAGGSGVIL-NHSEAPLK--------LNDLARLVAKAK---SLGLDVVV-CAPDPRTSLAAAALGPHAVAVEPP-ELIGTGRAVSRYKPEAIVETVGLVSRHFPEVSVITGAGIESGDDVAAALRLGTRGVLLASAAVKAKDPYAKIVELAKPLSE----------------------------

General information: TITO was launched using: RESULT: Template: 1W0M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 1220 -15205 -12.46 -73.45 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain H : 0.67 3D Compatibility (PKB) : -12.46 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.368

(partial model without unconserved sides chains): PDB file : Tito_1W0M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1W0M-query.scw PDB file : Tito_Scwrl_1W0M.pdb: