Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDITLAPVQPDQPTHLMATYGRQAISFVRGRGAYLYTQDGTEYLDALTGIAVCGLGHTHPTIAEAIAEQAATLVHTSN--LFEIPWQTAAAQKLAEVSG--MEEIFFSNSGAESNEGAIKIARKFGTQQGISLPKIIVAEQSFHGRTLATLSATGNKKVQDGFAPLVEGFIRVPFGDIEAIQEAALQHPDIVAILIEPIQGEGGINTAPQGFSYLEEVRQICNQHNWLMMLDEIQTGNGRTGKYFAYQHTNIIPDVLTTAKGLGNG-FPVGAVMTQGKAVGLLGP-----GSHGSTYGGTVLGSRVVYTVIDTIQKENAVENAAVVGNYIVDQLRAQLSD---KNVQVRGFGMMIGIQLPKDC--------------------------------AELVAIARDEYKLIINVTA--GSVVRLLPPINMTQAQADILLECLVALITNYL 4PPM Chain:A ((414-848)) ----------------------RCDNVFRSAAGTQLYDDAGEAFLDMVAGYGCLNLGHNPQPVVNALKNYLDAQGPNFIQYISIPEQTAKLAEVLCRLAPGNMGRVFFSNSGTEAVEAAMKIAKAST-----GKPGIAYLRNSYHGKTLGALSITGRDKHRRYFTPLLDAMVEVPFGDLAALREALN-REDVGALMIEPIQGEGGVH--IPPAGYLQAVQQLCRETGVLLMVDEVQTGLGRTGKLFACEWDGIEPDVLMLSKSLSGGLIPIGATLCRADLWQKAYGTADRFLVHSSTYGGGNLASVVALSALREILAQDLVGHAERMGAYFKQALSE-IAARYPFVSEVRGRGLMLGIQFDQA-HTTWKFLPDPVQAHLRAAMDRMEQALGEMFCMKFVTKLCQD-HKILTFITANSSTVIRIQPPLIISKAEIDRFVGAFATVCEEL-

General information: TITO was launched using: RESULT: Template: 4PPM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1904 -4146 -2.18 -11.27 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -2.18 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.443

(partial model without unconserved sides chains): PDB file : Tito_4PPM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PPM-query.scw PDB file : Tito_Scwrl_4PPM.pdb: