Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------------MYSTHRSKTICSKM-ARAFPGYSVMFFMHKK-----------TLVLLLSTFC-------ASTYIHAKTDCYPSSPPVV-----KQLDQVYYGMNNLRIFYTTSSDNALTFQQDKNLNHIPDYVENIAIQANSIRKAWS--------VLGYQDPLNSP---RYKNDVKYIDIQIQKLNGLG-LAYDEPHRHINNPLKKNACALIILISNN------IPNLTTN---------------SSLIA-HELFHLY--TYGYTMFKQ--PWLTESLA------AWSEGIIRKDKLGIYGQRNLPENEEDFEK----YVLQRTYRTSQLWNRLTTLIDRSDGKLF--LPLSLMQQKYT--DGQPVFQDKYLKGTLFIRKFLENLGRQ---DQKISVLNNWNLYHWNEIDQNSAHFNEQILD---------TLLITLESTVERNIEKNK---------FKNLLKKIVKNKKTN------------------------------------- 5K5S Chain:A ((20-598)) YGPDQRAQKKGDIILGGLFPIHFGVAAKDQDLKSRPESVECIRYNFRGFRWLQAMIFAIEEINSSPALLPNLTLGYRIFDTCNTVSKALEATLSFVAQNKIPSTIAVVGATGSGVSTAVANLLGLFYIPQVSYASSSRLLSNKNQFKSFLRTIPNDEHQATAMADIIEYFRWNWVGTIAADDDYGRPGIEKFREEAEERDICIDFSELISQYSDEEEIQHVVEVIQNSTAKVIVVFSSGPDLEPLIKEIVRRNITGKIWLASEAWASSSLIAMPQYFHVVGGTIGFALKAGQIPGFREFLKKVHPRKSVHNGFAKEFWEETFNCHPLCTGDENINSVETPYIDYTHLRISYNVYLAVYSIAHALQDIYTCLPGRGLFTNGSCADIKKVEAWQVLKHLRHL-QFTNNMGEQVTFDECGDLVGNYSIINWHLSPEDGSIVFKEVGYYNVYAKKGERLFINEEKILWSGFSREVPFSNCSRDCLAGTRKGIIEGEPTCCFECVECPDGEYSDETDASACNKCPDDFWSNENHTSC

General information: TITO was launched using: RESULT: Template: 5K5S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2075 49383 23.80 134.56 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 23.80 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.122

(partial model without unconserved sides chains): PDB file : Tito_5K5S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5K5S-query.scw PDB file : Tito_Scwrl_5K5S.pdb: