Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGRKTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAVYQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQRNENPVKSGTGELTI 3SRU Chain:X ((3-152)) -LSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMGRKTFESIG-KPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLP----GHVFIFGGQTLFEEMIDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVASSVEGKLDEKNTIPHTF-------------------

General information: TITO was launched using: RESULT: Template: 3SRU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 686 -114987 -167.62 -766.58 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain X : 0.84 3D Compatibility (PKB) : -167.62 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.469

(partial model without unconserved sides chains): PDB file : Tito_3SRU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3SRU-query.scw PDB file : Tito_Scwrl_3SRU.pdb: