Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSRPCLLLLGIDVVFAHISGSCINHVGRADLVMLRRGFCRSIPLLGPTFTELVERLDSFEKEFIEWKAFRKAVESTSPKVTEAAKEIIAESMKEVKTSKVNLENPLTPEEFTELNQFYAKQKVKDIFFENLLYINTPNDLMQHSETVFRQYL-VRIARRVRHLNHAPYGLSQMPGIQMLKKWYQWSFHDVRS--TPVPTTRDECYRCDRMVRRVFLRHYNVSSLITDGMVEFASREGWTHVDEEVMRTYDELQNFFEAFCLGRVRLRFLVGNYMYLSTKILGVSKEEYAVNDPDGLTVPIFFDHNAEDFVGQICKKCSLLVLTKCAIKEAQ--------ASYDAEI-ELKLAGDPNLV-FVGIPYITYDIICAMLEDAVSANVDRQERTG-KACTKIEVTLAQWPTNKRFVLRISDTAGGMTLRQASKQLSCWSLYRNIQGHNQDTISTWTSS-PIRLPYAYNAARVIGGNITLASIEGYGTDRQLYLPSTGLAGVSL 5M4M Chain:A ((22-361)) -------------------------------------------------------------------------------------------------------------------EHFSKFSPSPLSMKQFLDFG-SNACEKTSFTFLRQELPVRLANIMKEINLLPDRVLSTPSVQLVQSWYVQSLLDIMEFLDKDPEDHRTLSQFTDALVTIRNRHNDVVPTMAQGVLEYKD----TYGDDPV--SNQNIQYFLDRFYLSRISIRMLINQHTLIF----------------D-------PKH-----IGSIDPNCNVSEVVKDAYDMAKLLCDKYYMASPDLEIQEINAANSKQPIHMVYVPSHLYHMLFELFKNAMRATVESHESSLILPPIKVMVALGE----EDLSIKMSDRGGGVPLRKIERLFSY--MYSTAP---------PLAGFGYGLPISRLYAKYFQGDLQLFSMEGFGTDAVIYL----------

General information: TITO was launched using: RESULT: Template: 5M4M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1279 -39902 -31.20 -129.55 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -31.20 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.431

(partial model without unconserved sides chains): PDB file : Tito_5M4M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5M4M-query.scw PDB file : Tito_Scwrl_5M4M.pdb: