Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQNVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVLGPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAK-AERLHGAHIYMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETITITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVEVIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFENRFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETVVGKLVHLDRGYYGFHEKLAQLGAKIQRIEANDEDE 2RL2 Chain:A ((1-420)) MDKFRVYG-QSRLSGSVNISGAKNAALPILFAAILATE-PVKLTNVPELKDIETTLKILRQLGVVVDRDATGAVL-LDASNINHFTAPYELVKTMRASIWALAPLVARFHQGQVSLPGGCSIGARPVDLHISGLEKLGADIVLEEGYVKAQVSDRLVGTRIVIEKVSVGATLSIMMAATLAKGTTVIENAAREPEIVDTADFLNKMGAKITGAGSAHITIEGVERLTGCEHSVVPDRIETGTFLIAAAISGGCVVCQNTKADTLDAVIDKLREAGAQVDVTENSITLDMLGNRPKAVNIRTAPHPGFPTDMQAQFTLLNMVAEGTSIITETIFENRFMHIPELIRMGGKAEIEGNTAVCHGVEQLSGTEVIATDLRASISLVLAGCIATGETIVDRIYHIDRGYEHIEDKLRGLGAKIERFSG-----

General information: TITO was launched using: RESULT: Template: 2RL2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2778 -157148 -56.57 -375.05 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : -56.57 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.501

(partial model without unconserved sides chains): PDB file : Tito_2RL2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2RL2-query.scw PDB file : Tito_Scwrl_2RL2.pdb: