Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQVELIQALPKAELHVHIEGTFEPELMFAIAQRNQIQIPYKSVEEVKQAYNFH---NLQSFLDIY-YAGANVLVHEQDFYDLAWAYFEKCAEDRVVHTEMFFDPQ------------THTDRGIAFATVINGLKRACADAKDKFGISSQLIMCFLRHLSEEAAFETLEQALPF-KEDIIAVGLDSSEVGHPPAKFE---RVFAKAREEGFLIVAHAGEEGPPEYVWEALDLLKVNRIDHGVRSEEDEQLMARLINEKMPLTVCPLSNLKLCVVKDMKDHNIRRLLQKGVHVTVNSDDPSYFGGYMNDNFFAIQQALDLTNDELKQLAINSFEASFISDEEKQKWISEIQKI 3MVI Chain:A ((7-332)) ----------PKVELHVHLDGAIKPETILYFGKKRGIALPADTVEELRNIIGMDKPLSLPGFLAKFDYYMPVIAGCREAIKRIAYEFVEMKAKEGVVYVEVRYSPHLLANSKVDPMPWNQTEGDVTPDDVVDLVNQGLQEGEQAFGIKVRSILCCMRH-QPSWSLEVLELCKKYNQKTVVAMDLAGDETIEGSSLFPGHVEAYEGAVKNGIHRTVHAGEVGSPEVVREAVDILKTERVGHGYHTIEDEALYNRLLKENMHFEVCPWSSYLTGAWDPKTTHAVVRFKNDKANYSLNTDDPLIFKSTLDTDYQMTKKDMGFTEEEFKRLNINAAKSSFL---------------

General information: TITO was launched using: RESULT: Template: 3MVI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1815 12100 6.67 39.54 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : 6.67 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.455

(partial model without unconserved sides chains): PDB file : Tito_3MVI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3MVI-query.scw PDB file : Tito_Scwrl_3MVI.pdb: