Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTAQIELDELSVGADYEKVANRFRPIFEKIAQGAIQREKERILPFEPIQWLKEAKLGAVRIPRKYGGDGVS-LPQLFQLLAELAEADSNIVQAL-----RGHFAFVEDRLVAHKEHSQEVWFQRFVQGDLVGNAWTEVGNVQIGDVVTRVTKDASGNLVVNGEKYYSTGSIFADWIDLFAYDEVND---RHVIAAIYRHETGVSVIDDWDGFGQKTTGSGTLKVHQVHLPASHLI---PFDQRFKYQTAFYQVVHLATL---TGIARAAVETFSQEIRERKRIFSHGNGDLVRHAPQ--VLQVVGKASAQAYASEVI---TLKTAEALQKAYESHFAESEVKEHQFNVDAELESAQGQVVISNLVLDLTSQLFNALGASASSQVKQLDRFWRNARTVSSHNPLIYKEKVIGDWEVNRTDLPFVWQIGASPRAKSA 3M9V Chain:A ((29-395)) ---------------------DRLAAHLPRIRSTAAEHDRDGTFPTDTFDALRKDGLMGATVPAELGGLGVDRLYDVAVALLAVARADASTALALHMQLSRGLTLGYEWR---HGDERARTLAERILRGMVAGDAVVCSGIKDHHTAVTTLRPDGAGGWLLSGRKTLVSMAPVGTHFVINARTDG--GPPRLASPVVTRDTPGFTVLDNWDGLGMRASGTVDIVFDDCPIPADHVLMRDP--ARNDAVLAGQTVSSVSVLGVYVGVAQAAYDTAVAALERRP------------EPPQAAALTLVAEIDSRLYALRATAGSALTAADAL-----SADLSGDMDERGRQMMRHFQCA--KLAVNRLAPEIVSDCLSLVGGASYTAGHPLARLLRDVQAGRFMQPYAYVDAV--------------------------

General information: TITO was launched using: RESULT: Template: 3M9V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1785 1824 1.02 5.32 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 1.02 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.359

(partial model without unconserved sides chains): PDB file : Tito_3M9V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3M9V-query.scw PDB file : Tito_Scwrl_3M9V.pdb: