Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPRIPVINTSHLDRIDELLVDNIDTGEFKLHRSVFTDEALFDLEMKYIFEGNWVYLAHESQIPNNNDYYTTYIGRQPIIIARNRNGELNAMMNACSHRGAQLCRYKRGNKATYTCPFHGWTFNNSGKLLKVKDPTDAGYSDCFNQDGSHDLKKV------ARFESYKGFLFGSLNPDVPSLEEFLGETTKIIDMIVDQSEHGLEVLRGSSTYTYEGNWKLTAEN-GADGYHV-SAVHWN--YAATTQHRKETQA-----ADNIRAMSAGSWGKQGGGSYGFENGHMLLWTQWANPEDRPNFPKADEYTEKYGEAMSKWMIERSRNLCLYPNVYLMDQFGSQIRVLRPLSVNRTEVTIYCI----AP---KGEARARRIRQYEDFFNASGMATPDDLEEFRACQAGYAGIALEWNDMCRGSKHWIYGPDDAANEIGLKPMLSGIKTEDEGLYLAQHQYWLKTMKHAIATEKEIADQGETA 2YFL Chain:A ((28-401)) ----------------------VDQEKGLLDPRIYADQSLYELELERVFGRSWLLLGHESHVPETGDFLATYMGEDPVVMVRQKDKSIKVFLNQCRHRGMRICRSDAGNAKAFTCSYHGWAYDIAGKLVNVPFEKEA---F---------FDKAEWGPLQARVATYKGLVFANWDVQAPDLETYLGDARPYMDVMLDRTPAGTVAIGGMQKWVIPCNWKFAAEQFCSDMYHAGTTTHLSGILAGIPPEMDLSQAQIPTKGNQFRA----AWGGHGSGWYVDEPGSLLAVMGPKVTQYWTEGPAAELAEQRLGHTGMPVRRMVGQHMTIFPTCSFLPAM-NQIRVWHPRGPNEIEVWAFTLVDADAPAEIKEEYRRHNIRN----FSAGGVFEQDDGENWVEIQKGLRG-----------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2YFL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1754 -27697 -15.79 -80.75 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -15.79 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.406

(partial model without unconserved sides chains): PDB file : Tito_2YFL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YFL-query.scw PDB file : Tito_Scwrl_2YFL.pdb: