Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVSNFLLLSKEMNMAIYVDNMRIKHKGKEWCHLMADSLEELHDFALTLGVKRCWFHKNASYPHYDVTVEV-RNRALIYGAVPAERKKII-ECGRKLRIELLNNNQINRYNKLQIELF 1PAM Chain:A ((583-686)) -------TGDQVTVRFVINNATTALG--QNVFLTGNV-SELGNWDPNNAIGPMYNQVVYQYPTWYYDVSVPAGQTIEFKFLKKQGSTVTWEGGANRTFTTPTSGTATVNVNWQP---

General information: TITO was launched using: RESULT: Template: 1PAM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 495 -42560 -85.98 -417.25 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -85.98 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.224

(partial model without unconserved sides chains): PDB file : Tito_1PAM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1PAM-query.scw PDB file : Tito_Scwrl_1PAM.pdb: