Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIGQYRVTALIPARGGSKRLPRKNVKLLVDKPLIAWSIEVAKASKYIDRVVVSTDDEEIKQVSEQYGAEVPFLRPEYLSNDHASSFDVIKHAIDFLHLGQKNELIVLLQPTSPL---RLVSELDTALEFFIAKNAKGIVSISETEH--SPMWSNTLPE-NGCMSDFIRPEVQGKRS-----QDLPK------FFRLNGSIYIY----------------ETLCLLEQSKIFFNENVYGFETSLKT-AIDIDTDLDFLIAETIMKNRAIE 3DUV Chain:A ((4-250)) -------TVIIPAR----------------KPMIQHVFEKALQSG-ASRVIIATDNENVADVAKSFGAEVC-----MTSVNHNSGTERLAEVVEKLAI-PDNEIIVNIQGDEPLIPPVIVRQVADNLAKFNVNMASLAVKIHDAEELFNPNAVKVLTDKDGYVLYFSRSVIPYDRDQFMNLQDVQKVQLSDAYLRHIG-IYAYRAGFIKQYVQWAPTQLENLEKLEQLRVLYNGERIHVELAKEVPAVGVDT------AEDLEKVRAI-

General information: TITO was launched using: RESULT: Template: 3DUV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 903 -31903 -35.33 -161.94 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -35.33 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.343

(partial model without unconserved sides chains): PDB file : Tito_3DUV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DUV-query.scw PDB file : Tito_Scwrl_3DUV.pdb: