Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMDYQNAVQAFDLESTAKQMLSGSLDALNACYECCDRH--ADGDKIALYWQGKDGRKEQY-TFRELKEWSSQFANFLKSQGVKAGDRISGLLPRTPELIVTILAAWRIGAVYQPLFTAFGPKAIEHRIQLAQSKLVVT-DMGNRS--------KLDEIEKCPAIMTVADAQGTPLKAGDFNF-------WNE-VKQQSDQCDLVMRSIQDPFLLMFTSGTTG-PAKPLEVPLKALIAFGRYMQDAIGLTEEDSFWNIADPGWAYGLYYAITGPLFLGHATLFYEGGFSIDS---LCQIVKDYKVNNLAGAPTAYRMMMA-ADPAQMAPLKGQFRVVSSAGEPLNPEVIRWFKQVL---DAPIYDHYGQTEVGMVVCNHHGLKHEIHAGSAGFPSPGYRVAIVNEQGEELPPDTPGILAVDIS---QSPMMWFGGYKESRKSPF--IGHYYLTGDTAELHADGSMSFVGRSDDVITTSGYRIGPFDVESALLEHDAVIEAAVIGVPDPDRTEVVKAFVILAAGIQPSDALAEELSQFVKRRLSAHAYPRLVEFVSELPKTPSGKIQRFLLRNQEIAKQQAKAG 2P2F Chain:A ((74-621)) --------------------------TLNLAANCLDRHLQENGDRTAIIWEGDDTSQSKHISYRELHRDVCRFANTLLDLGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHSVIFGGFSPEAVAGRIIDSSSRLVITADEGVRAGRSIPLKKNVDDALKNPNVTSVEHVIVLKRTGSDIDWQEGRDLWWRDLIEKASPEHQPEAMNAEDPLFILYTSGSTGKPKGVLHTTGGYLVYAATTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVPNWPTPARMCQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINPEAWEWYWKKIGKEKCPVVDTWWQTETGGFMITPLPGAIELKAGSATRPFFGVQPALVDNEGHPQEGATEGNLVITDSWPGQARTL-FGDHERFEQTYFSTFKNMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHAIKGQAIYAYVTLNHGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILR------------

General information: TITO was launched using: RESULT: Template: 2P2F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3093 -45264 -14.63 -88.75 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -14.63 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.530

(partial model without unconserved sides chains): PDB file : Tito_2P2F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P2F-query.scw PDB file : Tito_Scwrl_2P2F.pdb: