Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDFNCDLVWMNSMSALLRFTQFVQKTFALWVIIFAALALWQPEFFVWLKAYIPWILGIIMLGMGMTMTVDDFKGVLQSPKAVLIGVVAQFVVMPGLAFILCKLFNLPPEIAVGVILVGCCPGGTASNVITYMAKGNVALSVACTSVSTLLAPVLTPAIFYLLASQWLKIDAASMFISILQVVLLPIVIGLILRTWLKRQVESYIQVMPLVSVIAIVAIVAAIIGGSKAAILQSGLLILAVVILHNGLGYLLGFAAARFFKLPYADSKAIAVEVGMQNSGLGVALAAVHFAASPITAVPSAIFSLWHNISGPALATYWAS---KHKQE 4N7W Chain:B ((5-306)) ----------------------ITRLFPVWALLLSVAAYFRPTTFTGIGPYVGPLLMLIMFAMGVTLRLDDFKRVLSRPAPVAAATFLHYLIMPLTAWILAMLFRMPPDLSAGMVLVGSVASGTASNVMIYLAKGDVALSVTISAVSTLVGVFATPLLTRLYVDATISVDVVGMLKSILQIVVIPITAGLVIHHTFTKTVKRIEPYLPAMSMVCILAIISAVVAGSQSHIASVGFVVIIAVILHNGIGLLSGYWGGKLFGFDESTCRTLAIEVGMQNSGLAATLGKIYF--SPLAALPGALFSVWHNLSGSLLAGYWSGKPVKKDQ-

General information: TITO was launched using: RESULT: Template: 4N7W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1677 -275260 -164.14 -920.60 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.84 3D Compatibility (PKB) : -164.14 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.431

(partial model without unconserved sides chains): PDB file : Tito_4N7W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4N7W-query.scw PDB file : Tito_Scwrl_4N7W.pdb: