Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKKGMINRELKREKTVAKYAVKRAELKATIANVNASDEERFEAMLKLQALPRNASPVRLRNRCGLTGRPHGYFRKFGLSRNKLRDTVMQGDVPGVVKASW 5AJ3 Chain:N ((36-128)) --------RDVKRRKMAYEYADERLRINSLRKNTILPKDLQEVADEEIASLPRDSCPVRIRNRCVMTSRPRGVKRRWRLSRIVFRHLADHGQLSGVQRAMW

General information: TITO was launched using: RESULT: Template: 5AJ3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain N - contact count / total energy / energy per contact / energy per residue : 228 -9837 -43.14 -105.77 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain N : 0.76 3D Compatibility (PKB) : -43.14 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.568

(partial model without unconserved sides chains): PDB file : Tito_5AJ3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5AJ3-query.scw PDB file : Tito_Scwrl_5AJ3.pdb: