Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSMSPSSSGHVNMMKGQPFHVKYREIIRRMSFLIGALLVFRLGAHIPVPGINNAALENLFHANQGTILGLFNMFSGGALERMSILALGIMPYISASIIVQLMS-TVIPSLEALKKEGEQGKRKINQYTRQGTLLLAVVQAVGMCAG---LIGQGITLSSGLAFYIPAVTSLVAGTMFLMWLGEQITERGIGNGISMIIFAGIVAGLPKLIMQSVSSVDNGQTSLIGLVIFGLLSLGVLAAIVFIEKAQRRIPVNYAQKQQGRRIFTAQQTHLPLKINMAGVIPAIFASSLLLFPASLGQWVGSADPNAGLIKRSLQDLALVLSPGQPLYLVLFGALIIFFCYFYTALVFSPKEVSENLKRSGAYVPGIRPGEQTARYLDHILNRLTFIGAIYIAVICLMPMILQSSFGIP--FYLGGTSLLIVVVVVMDFMAQLQAHLTFHQYDNQTLMRKTTAHPKG 5EUL Chain:Y ((13-419)) ------------------------DIRNKIIFTLLMLIVFRIGTFIPVPSVNTDVLK---LQDQLNAFGVLNIFCGGALQNFSIFAMGVMPYITASIIVQLLQMDVVPKFAEWSKQGEMGRRKLAQFTRYFTIVLGFIQALGMSYGFNNLAGGMLIQNP-IGTYLLIAVVLTAGTAFLMWLGEQITAKGVGNGISIIIFAGIVSGIPT-ILNQIYA-------IVRLLLVALAVVAVIVGVIYIQQAFRKIPIQ--------------STHLPLKVN-----PVIFAVSFLIAPPTIASFFGTND------RRTFDYT-------HPVGMTIYVVLIIAFTYFYAFVQVNPEQMADNLKKQGGYIPGIRPGKNTQEYVTRILYRLTLVGSLFLAFIAVLPVFFVNFANLPPSAQIGGTSLLIVVGVALETMKQLESQLVKRHY---------------

General information: TITO was launched using: RESULT: Template: 5EUL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain Y - contact count / total energy / energy per contact / energy per residue : 1469 -206898 -140.84 -560.70 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain Y : 0.80 3D Compatibility (PKB) : -140.84 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.398

(partial model without unconserved sides chains): PDB file : Tito_5EUL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EUL-query.scw PDB file : Tito_Scwrl_5EUL.pdb: