Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSSLSLLHPYPFEKLNQLFKDTTPANLPLIPLSIGEPKHPAPEFVKQAIIDNFNHLST-YPNSKGLPELRQSIADWLTKRFKLNSISAENHILPVSGTREGIFSFVQTLINREDAPYVVMPNPFYQIYEGAALLAGAKPYFINCTEENGYLGDFDAVPAEVWEKTALLFVCTPGNPTGTVLSKEQFKKLIALSDQYNFVIASDECYSELWFDQ---APTGLLEVCAELGRGDYKNCIVFHSLSKRSNLPGMRSGFIAGDAALLKPYLQYRTYHGAAMPVQHQLASIAAWNDE----SHVEENRKQYRAKFDLFQSELGHL-LPLQKPDAGFYYWLK---VDNDETFAKMLMEKAHIKVLPGRYLSRDTEQGNPGANHVRMALVADLAQCEEVVKRLKAIL 1BKG Chain:A ((33-382)) -----------------------------LVALTAGEPDFDTPEHVKEAARRALAQGKTKYAPPAGIPELREALAEKFRRENGL-SVTPEETIVTVGG-KQALFNLFQAILDPGDE--VIVLSPYWVSYPEMVRFAGGVVVEVETLPEEGFVPDPERVRRAITPRTKALVVNSPNNPTGAVYPKEVLEALARLAVEHDFYLVSDEIYEHLLYEGEHFSP----------GRVAPEHTLTVNGAAKAFAMTGWRIGYACGPKEVIKAMASVSSQSTTSPDTIAQWATLEALTNQEASRAFVEMAREAYRRRRDLLLEGLTALGLKAVRPSGAFYVLMDTSPIAPDEVRAAERLLEAGVAVVPGTDFA--------AFGHVRLSYATSEENLRKALERFARVL

General information: TITO was launched using: RESULT: Template: 1BKG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1993 -4575 -2.30 -13.54 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -2.30 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.558

(partial model without unconserved sides chains): PDB file : Tito_1BKG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1BKG-query.scw PDB file : Tito_Scwrl_1BKG.pdb: