Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNIYQFEAELLVGDIKQFADYKGKVLLIVNTASKCGFTP-QFAGLEKLYEKYKDLGLEVLGFPCNQFGGQDPGSNKEIGTFCQRNYGVKFPMFAKVDVKGPEAHVIFRYLTRE--AKGILGSSTIKWNFTKFLVGKDGSVLNRYAPTTKPEALEADIEKALAS 5H5S Chain:A ((12-165)) --SMHEFSAKDIDGHMVNLDKYRGFVSIVTNVASQ-GKTEVNYTQLVDLHARYAERGLRILAFPSNQFGKQEPGSNEEIKEFAA-GYNVKFDMFSKIEVNGDDAHPLWKWMKIQPKGKGILGNA-IKWNFTKFLIDKNGVVVKRYGPMEEPLVIEKDL------

General information: TITO was launched using: RESULT: Template: 5H5S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 771 -3631 -4.71 -24.20 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -4.71 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.546

(partial model without unconserved sides chains): PDB file : Tito_5H5S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5H5S-query.scw PDB file : Tito_Scwrl_5H5S.pdb: