Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQKKSVLFQQLLPVIKQYQQSGFTHEKIVELLKDQHDLNLVTVKTFKSYLYRYAKVNPAMSKNTATLQSMPTSREIKKSSKLEHVCYDIRGPVLRAANEMEEQGHKIIKLNIGNPAPFGFEAPQEIINDVALNLPNA-IGYVDSKGIFPARKAICQYYQQKGILNMHVNDVYIGNGVSELIVMAMQGLLDDGDEMLIPMPDYPLWTAAVNLSGGTAIHYKCDEENSWYPDIADIESKITSNTRGIVIINPNNPTGSVYPRHVLEQIVALAKKHDLILFADEIYDKIVYDGIEHVAVASLAGDQLCISFNGLSKAYRIAGFRSGWMAITGDKSRAADYIEGLDMLASMRLCA-NVQAQYAIQTALGGYQSINDLIRPGGRLY-EQRNIAWEMLNEIPGVSCVKPEGAMYCFPRLDPNIYPIEDDEKLMLDLLRAEKVLLVQGTGFNWPTPDHFRVVFLPAENELREAIGRLGRFLANRR 1GC3 Chain:A ((23-382)) -------------------------------------------------------------------------------------------------ALELRRQGVDLVALTAGEP---DFDTPEHVKEAARRALAQGKTKYAPPAGIPELREALAEKFRRENGLSVTPEETIVTVGGSQALFNLFQAILDPGDEVIVLSPYWVSYPEMVRFAGGVVVEVETLPEEGFVPDPERVRRAITPRTKALVVNSPNNPTGAVYPKEVLEALARLAVEHDFYLVSDEIYEHLLYEG-EHFSPGRVAPEH-TLTVNGAAKAFAMTGWRIGYAC--GPK----EVIKAMASVSRQSTTSPDTIAQWATLEALTNQEASRAFVEMAREAYRRRRDLLLEGLTAL-GLKAVRPSGAFYVL--MDTS--PIAPDEVRAAERLLEAGVAVVPGTDFA--AFGHVRLSYATSEENLRKALERFARVL----

General information: TITO was launched using: RESULT: Template: 1GC3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2072 -31138 -15.03 -87.22 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -15.03 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.534

(partial model without unconserved sides chains): PDB file : Tito_1GC3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GC3-query.scw PDB file : Tito_Scwrl_1GC3.pdb: