Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQTLIFMHETGKISIDFFKECIMQDIPMSRWLSPLMAFCLSFVIMATLAPTMGIQIDRQIDFWLLWFGTMLLLALPVCYLEIALAKRSKTTALNALSSLTREADSSPKWRVVGCLAVVFIPFLA------------------GNVLSTA------------SNILVAQ---FA---PSISSQII-------FVGLAVAALVLSFIP-----RQILILLMTLGVIASIVLANMM-G----S-----TLQPWHWTSV-EFKEWGNATVLALVASGLGLGLYW-QNSVGAVQAQEGATKTVLPIWLAQLIA--------VVAFGFFSL------------------------QAQLPVLTWI---------FTGVMTSALFVQLAREQLAQRQLMPVLQWVIIVVAIAVWAVPEVHN---LFTLIL-MQ--WGL-LICLIYAVFAGWIMKISHLRKSMNFSNE-LFYNLWRIAVRIVLPLSIIVAMIAVIGQSI 2QJU Chain:A ((25-466)) ----------------------------------------------LRFPVQAA-ENGGGAFMIPYIIAFLLVGIPLMWIEWAMGRYGGAQGHGTTPAIFYLLWRNRFAKILGVFGLWIPLVVAIYYVYIESWTLGFAIKFLVGLVPEPPPTDPDSILRPFKEFLYSYIGVPKGDEPILKPSLFAYIVFLITMFINVSILIRGISKGIERFAKIAMPTLFILAVFLVIRVFLLETPNGTAADGLNFLWTPDFEKLKDPGVWIAAVGQIFFTLSLGFGAIITYASYV--RKDQDIVLSGLTAATLNEKAEVILGGSISIPAAVAFFGVANAVAIAKAGAFNLGFITLPAIFSQTAGGTFLGFLWFFLLFFAGLTSSIAIMQPMIAFLEDELKLSRKHAVLWTAAIVFFSAHLVMFLNKSLDEMDFWAGTIGVVFFGLTELIIFFWIFGADKAWEEINRGGIIKVPRIYYYVMRYITPAFLAVLLVVWARE--

General information: TITO was launched using: RESULT: Template: 2QJU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1504 -163703 -108.85 -494.57 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -108.85 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.189

(partial model without unconserved sides chains): PDB file : Tito_2QJU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QJU-query.scw PDB file : Tito_Scwrl_2QJU.pdb: