Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGQIPLEHGLIVATILFALGFYGVMV-RRNLLFMLMSLEIMMNAAALAFVLAGSVWAQPDGQVMFILILTLAAAEACIGLAIVLQFYHRFHHLDVDAASEMRG 5LC5 Chain:K ((5-96)) ---------YMNIMMAFTVSLVGLLMYRSHLMSSLLCLEGMMLSLFVMAALTILNSHFTLASMMPIILLVFAACEAALGLSLLVMVSNTYGTDYVQNLNLL--

General information: TITO was launched using: RESULT: Template: 5LC5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain K - contact count / total energy / energy per contact / energy per residue : 193 -39533 -204.83 -434.42 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain K : 0.73 3D Compatibility (PKB) : -204.83 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.173

(partial model without unconserved sides chains): PDB file : Tito_5LC5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5LC5-query.scw PDB file : Tito_Scwrl_5LC5.pdb: