Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKNCGSGQSKLKALRMHIYFEVDFQEQAQHYQAVLHSRGVTVDLQPIEKLNARFLRLNPDLALCVDENGLWLSA--NGMKMQPDWKA--EIPRLKRASLKSEMIARACQLGEKPVLVDATAGLGHDSLLMAYLGAQIQLVERHPILFTLLEDSKAQAQRDPFLSQFMDRIQLIFADSASYLKQLDQEEKTVDVVYLDPMFPQRDQNQQAIKKQAQVKKQMQLLHLLLPEDGEMDLGGHLLELAK-KVAKRVIVKRPRHAIFLANQEPAHQWQGDACRFDAYFQ 2R6Z Chain:B ((31-244)) ----------------------------------------------------------EQGSYLLAEHDTVSLRLVGEKSNVIVDFTSGAAQYRRTKG--GGELIAKAVNHTAHPTVWDATAGLGRDSFVLASLGLTVTAFEQHPAVACLLSDGIRRALLNPETQDTAARINLHFGNAAEQMPALVKTQGKPDIVYLDPMYP------------------MAYFHRLVGE--A-QDEVVLLHTARQTAKKRVVVKRPRLGEHLAGQAPAYQYTGKSTRFDVYLP

General information: TITO was launched using: RESULT: Template: 2R6Z.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 976 -30073 -30.81 -152.65 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain B : 0.72 3D Compatibility (PKB) : -30.81 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.392

(partial model without unconserved sides chains): PDB file : Tito_2R6Z.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2R6Z-query.scw PDB file : Tito_Scwrl_2R6Z.pdb: