Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDEEDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYT---SDDGFSVVNYQAINDRLGTWEDIEEM-----RSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDTDTSSVTRPRTSPLLTTFIDKNG-------KEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLEKGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKDNISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLASHDGIGLN------PVRGI--LPEKTILE--LVEQLKKEGAR-VNYKKNPDGTTSPYEINATYLDALSSPTDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRND---YKGVEKTGENRKINRQKFLLADIEKELQTDPLRTA--VYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEEKVLVIHNFS---NQSAGYLLPKGDY--LDLFSGQIIHCSQERKLIFQPYEYMWLKIIK 5N7J Chain:A ((130-627)) ---------------------------------------------------------------------------------LHLMPLFKCPEGKSDGGYAVSSYRDVNPALGTIGDLREVIAALHEAGISAVVDFIFNHTSNEHEWAQRCAAGDPLFDNFYYIFPDRRMPDQYDRT----LKEVFPDQHPGGFSQLEDGRWVWTTFNSFQWDLNYSNPWVFRAMAGEMLFLANLGVDILRMDAVIYIWKQMGTSCINLPQAHALIRAFNAVMRIAAPAVFFKSEA-ITHPDQVVQY-IGQDECQIGYNPLQMALLWNTLATREVNLLHQALTYRHNLPEHTAWVNYVRSHDDIGWTFADEDAAYLGISGYDHRQFLNRFFVNRFDGSFARGVPFSYDP--STGDARVSGTAAALVGLAQDDPH-AVDRIKLLYSIALSTGGLPLIYLGDEVGTLNDDDWSQDSNKSDDSRWAHRPRYNEALYAQ--RNDPSTAAGQIYQDLRHMIAVRQSNPRFDGGRLVTFNTNNKHIIGYIR---NNALLAFGNFSEYPQTVTAHTLQAMPFKAHDLIGGKTVSLNQD--LTLQPYQVMWLEI--

General information: TITO was launched using: RESULT: Template: 5N7J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2481 21784 8.78 47.15 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 8.78 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.382

(partial model without unconserved sides chains): PDB file : Tito_5N7J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5N7J-query.scw PDB file : Tito_Scwrl_5N7J.pdb: