Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPVVHAFRASQSGLDAGAMKRSSDSHSPPFFFLSSACLAYRTSPMPSPRFARPELNGIITVRRFQRPQLETEAPIRKGDLKEPLEAVAGRVGLRARCSTSAPSADLCLVDDHELVLMGKLVDKGVYYITGGVCIDAEGSDTSSQSPTAALVSSTACGAAPQPPDITVGWCFDARMLLHRSDMN-RSPETPHRLQRAIETLQGCERALDVLPVELLAPFTVHKDSSSGSDLGRAVPPISAANRSQWIPARLATYDEVCSFQDPQVYEHFLKSGAAL----VNLKSD----VYCNDETSSVAVRLSAGAVIDASVAALRGVAASRSGTASSAAGRVHPLVSFCLVRPPGHHCTASQPSGFCLVNNVAIAVQQLRIRHASALPSGPPRIAILDLDVHFGEGTASFVEGACDPASLLYLSLHRYDKRQFYPFDGRGDTAYVGGSRHAASKGSICNVAVHTNGQQPARCEQVISDHLMNSVLEEIFLPRLAEFGPDLVMVSLGFDAAYGDPLGKMAVE-GGFASVLSRLKWWCLHDGRTAGLVVVLEGGYNPEAVAQGV---LSVALALSLPRTDPL----------LQRFLEEKSPKVWSDLRQRQERRHREWQQLREERAEEDVGAAPSGLGSELKPAASDEPEQVQEDALLLDRHKRWCAALVAKVQQIHREAMTRER 5EFG Chain:D ((8-364)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------P-----ITGLVYDQRMMLHHNMWDSHHPELPQRISRI---------------------FSRHEE-------------LRLLSRCHRIPARLATEEELALCHSSK-HISIIKSSEHMKPRDLNRLGDEYNSIFISNESYTCAL-LAAGSCFNSAQAILTGQV--RNAVA--------------IVRPPGHHAEKDTACGFCFFNTAA-----LTARYAQSITRESLRVLIVDWDVHHGNGTQHIFE---EDDSVLYISLHRYEDGAFFPNSEDANYDKVG-----LGKGRGYNVNIPWNGGKMGDPEYMAAFH-------HLVMPIAREFAPELVLVSAGFDAARGDPLGGFQVTPEGYAHLTHQLM--SLAAGR---VLIILEGGYNLTSISESMSMCTSMLLGDSPPSLDHLTPLKTSATVSINNVLRAHAP-FWSSLR-----------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5EFG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 2159 -16510 -7.65 -49.43 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain D : 0.67 3D Compatibility (PKB) : -7.65 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.450

(partial model without unconserved sides chains): PDB file : Tito_5EFG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EFG-query.scw PDB file : Tito_Scwrl_5EFG.pdb: