Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTEVGPDDNVVNSFIITPPSPKSEWTIHDFELLHKLGGGNYGDVYLASVRKSNYVVAIKKLSIKKLAEFDIVNQLRREIEIAFNTRHKYLLRTYAYFFDEHDIYLILEPCSNGMLYSELNRVKMFPPPTAARYVAQLAEALLYLHQHHILHRDIKPENILLDHNQNIKLADFGWSVHDPLNRRKTSCGTPEYFPPEIVSRQIYDMSADLWCLGIFCFELLVGHTPFVSKDNDQIYKKIHAMQYTIPDSVPPEAKDLISNLLIREGSKRLALHRVLSHPFLLKYYYVPNGITPPTGKRPRS 2X6D Chain:A ((8-265)) -----------------------RQWALEDFEIGRPLGKGKFGNVYLAREKQSKFILALKVLFKAQLE----EHQLRREVEIQSHLRHPNILRLYGYFHDATRVYLILEYAPLGTVYRELQKLSKFDEQRTATYITELANALSYCHSKRVIHRDIKPENLLLGSAGELKIADFGWSVHAPSSR----CGTLDYLPPEMIEGRMHDEKVDLWSLGVLCYEFLVGKPPFEANTYQETYKRISRVEFTFPDFVTEGARDLISRLLKHNPSQRPMLREVLEHPWI--------------------

General information: TITO was launched using: RESULT: Template: 2X6D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1264 -178916 -141.55 -715.66 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -141.55 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.500

(partial model without unconserved sides chains): PDB file : Tito_2X6D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2X6D-query.scw PDB file : Tito_Scwrl_2X6D.pdb: