Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNVPHKSSLPEGIRPGTVLRIRGLVPPNASRFHVNLLCGEEQGSDAALHFNPRLDTSEVVFNSKEQGSWGREERGPGVPFQRGQPFEVLIIASDDGFKAVVGDAQYHHFRHRLPLARVRLVEVGGDVQLDSVRIF 2GAL Chain:A ((3-135)) ---VPHKSSLPEGIRPGTVLRIRGLVPPNASRFHVNLLCGEEQGSDAALHFNPRLDTSEVVFNSKEQGSWGREERGPGVPFQRGQPFEVLIIASDDGFKAVVGDAQYHHFRHRLPLARVRLVEVGGDVQLDSVRIF

General information: TITO was launched using: RESULT: Template: 2GAL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 666 -81497 -122.37 -612.76 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.99 3D Compatibility (PKB) : -122.37 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.99 QMean score : 0.700

(partial model without unconserved sides chains): PDB file : Tito_2GAL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GAL-query.scw PDB file : Tito_Scwrl_2GAL.pdb: