Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSVGFIGAGQLAFALAKGFTAAGVLAAHKI---MASSPDMDLATVSALRKMGVKLTPHNKETVQHSDVLFLAVKPHIIPFILDEIGADIEDRHIVVSCAAGVTISSIEKKLSAFRPAPRVIRCMTNTPVVVREGATVYATGTHAQVEDGRLMEQLLSSVGFCTEVEEDLIDAVTGLSGSGPAYAFTALDALADGGVKMGLPRRLAVRLGAQALLGAAKMLLHSEQHPGQLKDNVSSPGGATIHALHVLESGGFRSLLINAVEASCIRTRELQSMADQEQVSPAAIKKTILDKVKLDSPAGTALSPSGHTKLLPRSLAPAGKD 5BSF Chain:A ((15-276)) -TLGFIGAGKMAESIAKGAVRSGVLSPSRIKTAIHSNP----ARRTAFESIGITVLSSNDDVVRDSNVVVFSVKPQLLKDVVLKLKPLLTKDKLLVSVAAGIKM----KDLQEWAGHERFIRVMPNTAATVGEAASVMSLGGAATEEDANLISQLFGSIGKIWKADDKYFDAITGLSGSGPAYIYLAIEALADGGVAAGLPRDLALSLASQTVLGAASMATQSGKHPGQLKDDVTSPGGTTIAGVHELEKAGFRGILMNAVVAAAKRSQEL---------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5BSF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1105 -63028 -57.04 -243.35 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -57.04 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.344

(partial model without unconserved sides chains): PDB file : Tito_5BSF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5BSF-query.scw PDB file : Tito_Scwrl_5BSF.pdb: