Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIHFSINKNLFLQALNITKRAISSKNAIPILSTVKIDVTNEGVTLIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQNQIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRPILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDDI-ETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSMERARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYLIDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN 3T0P Chain:A ((11-366)) ----------LLKGVNIVSKAVPTRTTMAILECILIDASANEIKLMANDMELGIETIIDGTIEERGI-------IALDAKIFSEIVRKLPDNDVTIETDASFKTVISCEKAKFNIIGKSGDDFSYIPYVERNESIVLSQFTLKEVIRQTIFSIADNDNNKLMTGELFEIEENK-LRVVSLDGHRISIRYIEMKNHYDSKKVVVPGKTLQEISKIIPGSADEDVVIYITNNHIVFEFENTTVVSRLIEGEYFKIDQMLSSDYDTKVRINKRELLDCIDRATLLVKE-DKKPIIMNITDGNMELRINSF-IGSMNEDI-DIDKDGKDIMIGFNPKFFIDALRVIDEEEVNLYMVNPKAPCFI--KDDEGKFIYLILPVNF-

General information: TITO was launched using: RESULT: Template: 3T0P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1863 -104099 -55.88 -294.06 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -55.88 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.560

(partial model without unconserved sides chains): PDB file : Tito_3T0P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3T0P-query.scw PDB file : Tito_Scwrl_3T0P.pdb: