Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLMERNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNEHNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASISGAEGGCQAEVGSA-SAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLIC-DPVAGLVEVPCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLATTPTGRRLQKEIFGE 2HP0 Chain:B ((123-242)) -----------------------------------------------------------------------------------------------------------------------SLVPAIF-AVGEHVGANGHNCLKSYVLGIEVVATLGRAVGKGHYLAGWH-PTSTLGVFGATTAAALLLGADEEQLRNAWGIAASNSCGIIKNFGTM-----TKPMHTGSAARNGVLSAWLSMQSFT-----------------------------------------------

General information: TITO was launched using: RESULT: Template: 2HP0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 530 -66657 -125.77 -569.72 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.60 3D Compatibility (PKB) : -125.77 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.392

(partial model without unconserved sides chains): PDB file : Tito_2HP0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HP0-query.scw PDB file : Tito_Scwrl_2HP0.pdb: