Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKMERNINMKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKTSAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAGDDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAFIDKEGKLVKTHPGFMEKDAILQTLKELS 2H1B Chain:A ((2-129)) --------------------------------------------------------EGSDAPNFVLEDTNGKRIELSDLKGKGVFLNFWGTWCEPCKKQFPYMANQYKHFKSQGVEIVAVNVG----ESKIAVHNFMKSYGV-NFPVVLDTDRQVLDAYDVSPLPTTFLINPEGKVVKVVTGTMTESMI--------

General information: TITO was launched using: RESULT: Template: 2H1B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 600 -81906 -136.51 -639.89 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -136.51 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.682

(partial model without unconserved sides chains): PDB file : Tito_2H1B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2H1B-query.scw PDB file : Tito_Scwrl_2H1B.pdb: