Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------MLKRFLALVWLRCQIILSNKSILLQVLVPFAFTYFYKYLMETQGKVNDQQALVLLMMCLPFSLALAVGSPITI-----------ILSEEKEKYNLQTLLL--------SGVKGSEYILSTMF-------------------------LPFLLTFVIMGTTPLILGVTIVH---TFNYITIV---LLTSLSIILFYLLIGLTAKSQVVAQVISLPA--MILVAFLPMLSSLDKTVAKITDYSFMGLFTKF-----------FTKWEGFSWNE--TLIPNLTLLI--------WIVLLLTLITITIRKKKIS------------------------------- 4FFL Chain:A ((1-363)) MKTICLVGGKLQGFEAAYLSKKAGMKVVLVDKNPQALIRNYADEFYCFDVIKEPEKLLELSKRVDAVLPVNENLACIEFLNSIKEKFSCPVLFDFEAYRISRDKKKSKDYFKSIGVPTPQDRPSKPPYFVKPPCESSSVGARIIYGLEPDTLVEEYVEGEVVSLEVVGDGSHFAVVKETLVHIDETYDCHMVTPLPANPLFRQISHDLAANLPLKGIMDVEAIFGPKGLRVIEIDARFPSQTPTVVYYSSGINLIELLFRAFTDGVEEIENKYCIYEHLMFGENGVLIPVGEQVLSMGSDYGKFYEEPGIEIFLCKGEYPVFTMVFWGKDREETGAKRCKGLSVLKERFGAVL

General information: TITO was launched using: RESULT: Template: 4FFL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1171 -81492 -69.59 -343.85 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -69.59 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.132

(partial model without unconserved sides chains): PDB file : Tito_4FFL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FFL-query.scw PDB file : Tito_Scwrl_4FFL.pdb: