Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKDVSLFLLKKVFKSRLNWIILLLFASVLGVTFYLNSQTANSVSLERELETRLVDRERVINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLLKEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLEFPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNH-LDTAHLYPVSKVTFAMSSLGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKMQDVLFPSLLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGI-----V-GLLFGIQ-TIQP-LQRIAHLIPFTYLRSVEILS--GRLSKQIDN-----------------VDLNWSMGMVLLPCLIILLLVGILFIERWGSSRKKEVFKV 5DO7 Chain:C ((444-649)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VLRAVSDQESQDGLYQKWQMMLAYALHVLPFSVVATMIFSSVCY----WTLGLH---PE-----V-----------ARFGYFSAALLAPHLIGEFLTLVLLGIVQNPNIVNSVVALLSIAGVLVGSGFLRNIQEMPIPFKIISYFTFQKYCSEILVVNEFYGLNFTCGSMCAFTQGIQFIEKTCPGATSRFTMNFLILYSFIPALVILGIVVFKIRDHLI------

General information: TITO was launched using: RESULT: Template: 5DO7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 525 -87418 -166.51 -517.26 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain C : 0.55 3D Compatibility (PKB) : -166.51 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.239

(partial model without unconserved sides chains): PDB file : Tito_5DO7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DO7-query.scw PDB file : Tito_Scwrl_5DO7.pdb: