Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------MKRIIPVY---------IFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLISFL-WMYGQRKQAHKVNMKSRMKWLGVEFVSLLI---------ISLCFSLIHAQG------STNQANLIGLQHQIPWFSFLLFLINASMVEEFLYREILCNL----------VRKLDIRV-ALTSVLFALAHHTG----TILAWCLYVSLGMFL---GMVRYKSDLW-------GSMGL---------------HLVWNLLVYSLLLF----- 4P4M Chain:A ((92-378)) KPKLKAIQELTQVHGFGPRAAAALFDREGIFTVDELLQKADSIPSLTDQQRVGIKYFYDINEKIPMQESVLHENYLREKCMEVLGKDFSILICGSYRRRHPFSGDVDAILSRTLDAPPLSEPVAATGVLGHFVEFLESLKY-LEATMAQGPLKYMGMGRLPPRINTKVYKARRVDIRLIETKSVPTAMLTFTGSKNFNVIMRQAAISKGYLLNEYGLFKLGTPEEARGKNAGEELGVPKDELEDKRVEVRSEQDVFDVLGMPYAKPENRDP

General information: TITO was launched using: RESULT: Template: 4P4M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 810 -66688 -82.33 -343.75 target 2D structure prediction score : 0.34 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -82.33 2D Compatibility (Sec. Struct. Predict.) : 0.34 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.160

(partial model without unconserved sides chains): PDB file : Tito_4P4M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4P4M-query.scw PDB file : Tito_Scwrl_4P4M.pdb: