Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIID-SLPQLGGQPAILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTSKGSHLTKTVIIAMGGGAFKPRPLELEGVEDY--ENIHY-HVSNIQQYAGKKVTILGGGDSAVDWALAFEKIAPT-TLVHRRDNFRALEHSVQ-ALQESSVTIKTPFAPSQLLGDGKTLDKLEITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYAIGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL 5MJK Chain:A ((25-323)) ----YDVVIIGSGPAGMTAAMYTARSEMKTLLLERGVP--GGQ---MNNTAEIENYPGYETIMGPELSMKMAEPLEGLGVE-NAYGFVTGIEDHGDYKKIITEDDEFITKSIIIATGA---NHRKLEIPGEEEYGARGVSYCAVCDGAFFRNQEILVIGGGDSAVEEALYLTRFGQSVTIMHRRDKLRAQEIIQQRAFKEEKINFIWDSVPMEIKGDDKKIQSVVYKNVKTGEVTEKAFGGIFIYVGLDPVAEFVSDLGITDEAGWIITDDHMRTNIPGIFAVGDVRQKDFR--QITTAVGDGAQAAQEAYKFV--------------

General information: TITO was launched using: RESULT: Template: 5MJK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1722 44832 26.03 153.01 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 26.03 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.526

(partial model without unconserved sides chains): PDB file : Tito_5MJK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MJK-query.scw PDB file : Tito_Scwrl_5MJK.pdb: