Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEAGVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQ--LSEEELEHFVSSMTHFGYEKEELPGVVSDYIKGV 2EK5 Chain:A ((4-106)) ---------PLYKQIASLIEDSIVDGTLSIDQRVPSTNELAAFHRINPATARNGLTLLVEAGILYKKRGIGMFVSAQAPALIRERRDAAFAATYVAPLIDESIHLGFTRARI-----------

General information: TITO was launched using: RESULT: Template: 2EK5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 296 -41313 -139.57 -409.03 target 2D structure prediction score : 0.81 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -139.57 2D Compatibility (Sec. Struct. Predict.) : 0.81 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.669

(partial model without unconserved sides chains): PDB file : Tito_2EK5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EK5-query.scw PDB file : Tito_Scwrl_2EK5.pdb: