Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGLGGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFKLLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGD-TIVPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLRGAHTSTTKFDPEVFLAVD----CSP-AGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTTAEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQAFLQALVKKLDRSTVDLIKHY 2WYR Chain:A ((2-332)) -MSMIEKLKKFTQIPGISGYEERIREEIIREIKDFADYKV-DAIGNLI-VELGEG--EERILFMAHMDEIGLLITGITDEGKLRFRKVGGIDDRLLYGRHVNVVTEKGILDGVI-GATPPHL-----KSVIPWY-DLVIDIGAESKEEALEL-VKPLDFAVFKKHFSVL--NGKYVSTRGLDDRFGVVALIEAIKDLVDHELEGKVIFAFTVQEEVGLKGAKFLANHYYPQYAFAIDSFACCSPLTGDV-----KLGKGPVIRAVDNSAIYSRDLARKVWSIAEKNGIEIQIGVTGGGTDASAFQDRS---KTLALSVPIKYLHSEVETLHLNDLEKLVKLIEALAFEL-----------

General information: TITO was launched using: RESULT: Template: 2WYR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1893 -55876 -29.52 -174.61 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -29.52 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.508

(partial model without unconserved sides chains): PDB file : Tito_2WYR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2WYR-query.scw PDB file : Tito_Scwrl_2WYR.pdb: