Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQKMN---HLDVGEFVLLLPEHLRSEEEHYKSVFEDDLTSRISSQDERQQMTATVGYLESGQDRFVYNTT--PISYQQFLKDP-----IIIVITPQSTGPQSILFWI---DAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNY---------ITLLDNIQRERWVMLAGAVLGIATSILLFNTMNRLYFEEFRRAIFIKR------IAGLRFL------------------------EIHRTYLFAQLGVFLL---------GF--------VASVFLQVEI-------GVAFLVLLLFTGLSLLQLHVQ----------------MQKENKMSMLVLKGG 5HC3 Chain:A ((22-365)) MTNKIAEDPRID-PRIKAIFSGMDLGGGGDVES--REAMLEAASSEEATAVRDGLRVFLDACDNEEIAPSAGLKIEDYEFTSEPDGNIAKIQYIRPDSTDKLPCVYYIHGGGMQSLSCYYGNYRAWGKIIASNGVAVAMVEFRNALVPSALPEVAPYPAGLNDCVSGVKWVAAHADELGIDASRIIIAGESGGGNLTLAAGLRLKQEGSQDLIQGLYALCPYIAGSWPSEDSPSSTENNGILLDLHNNQGAMGYGIEAYEMRDPLAWPGFATEEDVSGLVPTFISVNECDPLRDEGINFYRLLLRAGVSAKCRQVMGTIHGTEIFPIACPDVSRDTAASIANFCKGG

General information: TITO was launched using: RESULT: Template: 5HC3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1237 -38899 -31.45 -154.36 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -31.45 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.273

(partial model without unconserved sides chains): PDB file : Tito_5HC3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HC3-query.scw PDB file : Tito_Scwrl_5HC3.pdb: