Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVDRVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLLDMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVEENSTIVNLLTNTEN--ADKISVKIQ--HGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGDKVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS 2QF4 Chain:A ((4-166)) ------------------------------------------------------------------------------------------------------------QATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVEENSTIVNLLTNTENADKIS--VKIQHGSTT--IYGIIIGYDKENDVLKISQLNSNSDISAGDKVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGN-

General information: TITO was launched using: RESULT: Template: 2QF4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 854 29888 35.00 187.97 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : 35.00 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.688

(partial model without unconserved sides chains): PDB file : Tito_2QF4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QF4-query.scw PDB file : Tito_Scwrl_2QF4.pdb: