Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPIDTQLTTLFCLIDDFCTDITQNVEQYMLTYGQTKRLRQSKIHASEVITLLLWFHLTGSRNFKAFYLYWAKPFLCSYFTNLPSYSRFIELKAKYAMYFVALIESLKVHSAGIAFIDSTKLAVCHNKRIHQHRVFADSASRGKTSVDWFYGFKIHLICDHIGRLVSYCITTGNVDD-RKVLPDLIEHSKLKGKL-FGDRGYVGKNWKSRLAEVGVQLITRVKRNMKPQ--VLAPFDHAVLKKRGIIEAPFKLMKSQFDLEHSRHRSKMGLLTTIFAALTLYALVLVNGYKSGIQQILKPIDLNSA 5LSJ Chain:B ((2-174)) -----------------------------------------------------------------------------------------------------TISRVKLLDTMVDTFLQKLVAAGSYQRFTDC--YKCFYQLQPAMTQQIYDKFIAQLQTSIREEISDIKEEGNLEAVLNALDKIVEEGKVRKEPAWRPSGIPEKDLHSVMAPYFLQQRDTLRRHVQKQEAENQQLADAVLAGRRQVEELQLQVQAQQQAWQALHREQRELVAVLRE-----------------------------

General information: TITO was launched using: RESULT: Template: 5LSJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 161 -11562 -71.81 -68.41 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.55 3D Compatibility (PKB) : -71.81 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.201

(partial model without unconserved sides chains): PDB file : Tito_5LSJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5LSJ-query.scw PDB file : Tito_Scwrl_5LSJ.pdb: