Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNFDRNTPIAFLGIGLMGNRMASHLIQAGFQVAVWNRT-ASACEELIDIGAHALDLSNIGQYPLILTCLADDKAVQAVFDQIQTNLKAGQVIVDFSSLSVAATKALAQAAALQDVIWIDSPVSGGTAGAEQGTLVIFAGGDAQTIEALSPVYNVLSQRVTRMGDTGTGQATKICNQLIVAANSALIAEAVALADRAGVDTTLLAPALAGGFADSKPFQILAP-RMATHTFEPVQWKVQTLSKDLNNAVTLANNVNLDIPVAQKALLQLQTHQKNSFAEKDLATMIQVVEQ 2CVZ Chain:A ((4-282)) --------VAFIGLGAMGYPMAGHLARR-FPTLVWNRTFEKALRHQEEFGSEAVPLERVAEARVIFTCLPTTREVYEVAEALYPYLREGTYWVDATSGEPEASRRLAERLREKGVTYLDAPVSGGTSGAEAGTLTVMLGGPEEAVERVRP-FLAYAKKVVHVGPVGAGHAVKAINNALLAVNLWAAGEGLLALVKQGVSAEKALEVINASSGRSNATENLIPQRVLTRAF-PKTFALGLLVKDLGIAMGVLDGEKAPSPLLRLAREVYEMAKRELGPDADHVEALRLLER

General information: TITO was launched using: RESULT: Template: 2CVZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1503 -35643 -23.71 -128.67 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -23.71 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.469

(partial model without unconserved sides chains): PDB file : Tito_2CVZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CVZ-query.scw PDB file : Tito_Scwrl_2CVZ.pdb: